Article
The roles of residues 5 and 9 of the recognition helix of Lac repressor in lac operator binding.
Journal of molecular biology - 20 Mar 1991
Sartorius J, Lehming N, Kisters-Woike B, von Wilcken-Bergmann B, Müller-Hill B
Abstract excerpt
We constructed expression libraries for Lac repressor mutants with amino acid exchanges in positions 1, 2, 5 and 9 of the recognition helix. We then analysed the interactions of residues 5 and 9 with operator variants bearing single or multiple symmetric base-pair exchanges in positions 3, 4 and 5 of the ideal fully symmetric lac operator. We isolated 37 independent Lac repressor mutants with five different amino...
Topics
- Amino Acid Sequence
- Amino Acids
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Gene Expression
- Gene Library
- Lac Operon
- Molecular Sequence Data
- Mutation