Article
Analysis of binding specificity of disulfide bonded dimeric lambda-Cro V55C protein with generic hexamer oligonucleotide microchip.
Journal of biomolecular structure & dynamics - 1 Dec 2003
Chechetkin V R, Prokopenko D V, Zasedateleva O A, Gitelson G I, Lomakin E S, Livshits M A, Malinina L, Turygin A Y, Krylov A S, Mirzabekov A D
Abstract excerpt
Binding specificity of mutant V55C disulfide bonded dimeric lambda-Cro protein (CroVC) to double-stranded DNA (dsDNA) was studied using generic hexamer oligonucleotide microchip. The curves of dissociation of hybridized DNA in the presence and absence of CroVC were converted into the effective discriminant constants to assess the relevant thermodynamic equilibrium binding constants for dsDNA-protein complexes....
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