Article
Mutant lambda repressors with increased operator affinities reveal new, specific protein-DNA contacts.
Genetics - 1 Jan 1992
Benson N, Adams C, Youderian P
Abstract excerpt
The binding specificities of four mutant lambda cI repressor proteins with increased affinities for operator DNA were examined. Two mutant repressors (Glu34----Lys and Glu83----Lys) have the same specificity of binding as wild-type repressor, whereas two (Gly48----Ser and Gly48----Asn) have new b...
Topics
- Bacteriophage lambda
- Base Sequence
- Binding Sites
- Consensus Sequence
- DNA, Viral
- DNA-Binding Proteins
- Escherichia coli
- Lysogeny
- Molecular Sequence Data
- Mutation
- Operator Regions, Genetic
- Repressor Proteins
- Transcription Factors
- Viral Proteins
- Viral Regulatory and Accessory Proteins