Symmetrical stabilization of bound Ca2+ ions in a cooperative pair of EF-hands through hydrogen bonding of coordinating water molecules in calbindin D(9k).

doi:10.1021/bi010551h

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Article

Symmetrical stabilization of bound Ca2+ ions in a cooperative pair of EF-hands through hydrogen bonding of coordinating water molecules in calbindin D(9k).

Biochemistry - 21 Aug 2001

Fast J, Håkansson M, Muranyi A, Gippert G P, Thulin E, Evenäs J, Svensson L A, Linse S

Abstract excerpt

Water molecules are found to complete the Ca2+ coordination sphere when a protein fails to provide enough ligating oxygens. Hydrogen bonding of these water molecules to the protein backbone or side chains may contribute favorably to the Ca2+ affinity, as suggested in an earlier study of two calbindin D(9k) mutants [E60D and E60Q; Linse et al. (1994) Biochemistry 33, 12478-12486]. To investigate the generality of...

Topics

Amino Acids
Animals
Binding Sites
Calbindins
Calcium
Cattle
Chelating Agents
Circular Dichroism
Crystallography, X-Ray
Dose-Response Relationship, Drug
Electrophoresis, Polyacrylamide Gel

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Hydrogen Bonding

Ions

Kinetics

Magnetic Resonance Spectroscopy

Models, Chemical

Models, Molecular

Mutation

Source

Open in PubMed

Identifiers

PMID: 11502182
DOI: 10.1021/bi010551h

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