Article
Characterization of the N-terminal half-saturated state of calbindin D9k: NMR studies of the N56A mutant.
Protein science : a publication of the Protein Society - 1 Jun 1995
Wimberly B, Thulin E, Chazin W J
Abstract excerpt
Calbindin D9k is a small EF-hand protein that binds two calcium ions with positive cooperativity. The molecular basis of cooperativity for the binding pathway where the first ion binds in the N-terminal site (1) is investigated by NMR experiments on the half-saturated state of the N56A mutant, which exhibits sequential yet cooperative binding (Linse S, Chazin WJ, 1995, Protein Sci 4:1038-1044). Analysis of...
Topics
- Animals
- Apoproteins
- Binding Sites
- Calbindins
- Calcium
- Cattle
- Helix-Loop-Helix Motifs
- Magnetic Resonance Spectroscopy
- Models, Molecular
- Mutation
- Protons