Article
A calbindin D9k mutant with reduced calcium affinity and enhanced cooperativity. Metal ion binding, stability, and structural studies.
Biochemistry - 18 Oct 1994
Linse S, Bylsma N R, Drakenberg T, Sellers P, Forsén S, Thulin E, Svensson L A, Zajtzeva I, Zajtsev V, Marek J
Abstract excerpt
In the native calcium-binding protein calbindin D9k (M(r) 8.700; 75aa; 2 EF-hands), the backbone carbonyl oxygen of Glu60 coordinates the Ca2+ ion in the C-terminal site (site II). The carboxylate group of the same residue forms a hydrogen bond to a water molecule that constitutes a Ca2+ ligand in the N-terminal site (site I). The mutant E60D, with the charge-conserving substitution Glu60-->Asp, has been prepared...
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