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Article

Disulfide bonds in homo- and heterodimers of EF-hand subdomains of calbindin D9k: stability, calcium binding, and NMR studies.

Protein science : a publication of the Protein Society - 1 Jun 1993

Linse S, Thulin E, Sellers P

Abstract excerpt

The effect of decreased protein flexibility on the stability and calcium binding properties of calbindin D9k has been addressed in studies of a disulfide bridged calbindin D9k mutant, denoted (L39C + P43M + I73C), with substitutions Leu 39-->Cys, Ile 73-->Cys, and Pro 43-->Met. Backbone 1H NMR assignments show that the disulfide bond, which forms spontaneously under air oxidation, is well accommodated. The...

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Identifiers

PMID: 8318902
DOI: 10.1002/pro.5560020612
PMCID: PMC2142409

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