Article
Functional properties of calbindin D9K mutants with exchanged Ca2+ binding sites.
The Journal of biological chemistry - 5 Jul 1990
Brodin P, Johansson C, Forsén S, Drakenberg T, Grundström T
Abstract excerpt
We have constructed three different engineered proteins based on calbindin D9K by either exchanging the two calcium binding sites within the protein or making the amino acid sequence of the two calcium binding sites identical. The individual calcium binding sites of the engineered proteins retain most of their ion binding characteristics as well as the basal structure of their Ca2+ ligand sphere in the new...
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