Functional properties of calbindin D9K mutants with exchanged Ca2+ binding sites.

Article

Functional properties of calbindin D9K mutants with exchanged Ca2+ binding sites.

The Journal of biological chemistry - 5 Jul 1990

Brodin P, Johansson C, Forsén S, Drakenberg T, Grundström T

Abstract excerpt

We have constructed three different engineered proteins based on calbindin D9K by either exchanging the two calcium binding sites within the protein or making the amino acid sequence of the two calcium binding sites identical. The individual calcium binding sites of the engineered proteins retain...

Topics

Binding Sites
Calbindins
Calcium
Magnetic Resonance Spectroscopy
Mutation
Recombinant Proteins
S100 Calcium Binding Protein G
Thermodynamics

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