Mutational effects on the cooperativity of Ca2+ binding in calmodulin.

doi:10.1021/bi00082a005

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Article

Mutational effects on the cooperativity of Ca2+ binding in calmodulin.

Biochemistry - 10 Aug 1993

Waltersson Y, Linse S, Brodin P, Grundström T

Abstract excerpt

The importance of the aspartate ligand in the +Y Ca2+ coordinating position of two EF-hands of calmodulin has been investigated. Synthetic calmodulin genes were used to produce engineered proteins with the wild-type bovine sequence as well as with aspartate 58 in Ca(2+)-binding site II and/or asp...

Topics

Allosteric Regulation
Allosteric Site
Amino Acid Sequence
Base Sequence
Calcium
Calmodulin
Escherichia coli
Models, Chemical
Molecular Sequence Data
Mutation
Peptide Fragments
Recombinant Proteins

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