Article
The Dictyostelium homologue of mammalian soluble adenylyl cyclase encodes a guanylyl cyclase.
The EMBO journal - 15 Aug 2001
Roelofs J, Meima M, Schaap P, Van Haastert P J
Abstract excerpt
A new Dictyostelium discoideum cyclase gene was identified that encodes a protein (sGC) with 35% similarity to mammalian soluble adenylyl cyclase (sAC). Gene disruption of sGC has no effect on adenylyl cyclase activity and results in a >10-fold reduction in guanylyl cyclase activity. The scg- null mutants show reduced chemotactic sensitivity and aggregate poorly under stringent conditions. With Mn(2+)/GTP as...
Topics
- Adenylyl Cyclases
- Amino Acid Sequence
- Animals
- Catalytic Domain
- Dictyostelium
- Guanylate Cyclase
- Humans
- Mammals
- Molecular Sequence Data
- Phenotype
- Phylogeny
- Sequence Homology, Amino Acid