Isolation of inactive and G protein-resistant adenylyl cyclase mutants using random mutagenesis.

doi:10.1074/jbc.270.39.22693

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Article

Isolation of inactive and G protein-resistant adenylyl cyclase mutants using random mutagenesis.

The Journal of biological chemistry - 29 Sept 1995

Parent C A, Devreotes P N

Abstract excerpt

We used random mutagenesis and phenotypic rescue of adenylyl cyclase-null Dictyostelium cells to isolate loss-of-function mutations in the enzyme. Mutants were (i) catalytically inactive or (ii) resistant to chemoattractant receptor and guanosine 5'-3-O-(thio)triphosphate stimulation. Both classe...

Topics

Adenylyl Cyclases
Amino Acid Sequence
Animals
Consensus Sequence
Conserved Sequence
Cyclic AMP
DNA Primers
Dictyostelium
Electrophoresis, Polyacrylamide Gel
GTP-Binding Proteins
Guanosine 5'-O-(3-Thiotriphosphate)
Guanosine Triphosphate

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Immunoblotting

Molecular Sequence Data

Mutagenesis

Phenotype

Polymerase Chain Reaction

Protein Structure, Secondary

Source

Open in PubMed

Identifiers

PMID: 7559391
DOI: 10.1074/jbc.270.39.22693

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7559391-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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