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Article

Isolation of inactive and G protein-resistant adenylyl cyclase mutants using random mutagenesis.

The Journal of biological chemistry - 29 Sept 1995

Parent C A, Devreotes P N

Abstract excerpt

We used random mutagenesis and phenotypic rescue of adenylyl cyclase-null Dictyostelium cells to isolate loss-of-function mutations in the enzyme. Mutants were (i) catalytically inactive or (ii) resistant to chemoattractant receptor and guanosine 5'-3-O-(thio)triphosphate stimulation. Both classes of mutants harbored substitutions within the cytoplasmic C1a domain. Mutations that inactivated the enzyme were often...

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Identifiers

PMID: 7559391
DOI: 10.1074/jbc.270.39.22693

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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