Article
In vivo site-directed mutagenesis using oligonucleotides.
Nature biotechnology - 1 Aug 2001
Storici F, Lewis L K, Resnick M A
Abstract excerpt
Functional characterization of the genes of higher eukaryotes has been aided by their expression in model organisms and by analyzing site-specific changes in homologous genes in model systems such as the yeast Saccharomyces cerevisiae. Modifying sequences in yeast or other organisms such that no heterologous material is retained requires in vitro mutagenesis together with subcloning. PCR-based procedures that do...
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