Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening.

Nucleic acids research - 1 Feb 2003

Young Lei, Dong Qihan

Abstract excerpt

Site-directed mutagenesis is an invaluable tool for functional studies and genetic engineering. However, most current protocols require the target DNA to be cloned into a plasmid vector before mutagenesis can be performed, and none of them are effective for multiple-site mutagenesis. We now describe a method that allows mutagenesis on any DNA template (eg. cDNA, genomic DNA and plasmid DNA), and is highly...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to Biotechnology
Share to DNA

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to DNA Primers

Share to Mutagenesis, Site-Directed

Share to Mutation

Share to Polymerase Chain Reaction

Share to Templates, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 12560514
DOI: 10.1093/nar/gng01
PMCID: PMC149223

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening.PMID 12560514Read ↗

A site-directed mutagenesis method particularly useful for creating otherwise difficult-to-make mutants and alanine scanning.Semantic proximity Read ↗

MALS: an efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR.Semantic proximity Read ↗

An In Vitro Single-Primer Site-Directed Mutagenesis Method for Use in Biotechnology.Semantic proximity Read ↗

Rapid and efficient site-specific mutagenesis.Semantic proximity Read ↗

Improved site-directed mutagenesis method using PCR.Semantic proximity Read ↗

PCR-based site-directed mutagenesis using primers with mismatched 3'-ends.Semantic proximity Read ↗

Mutagenesis of longer inserts by the ligation of two PCR fragments amplified with a mutation primer.Semantic proximity Read ↗

Site-directed mutagenesis.Semantic proximity Read ↗

A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method.Semantic proximity Read ↗

Multiple site-directed mutagenesis via simple cloning by prolonged overlap extension.Semantic proximity Read ↗

P3 site-directed mutagenesis: An efficient method based on primer pairs with 3'-overhangs.Semantic proximity Read ↗

A gradient PCR-based screen for use in site-directed mutagenesis.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.