Article
A simple vector modification to facilitate oligonucleotide-directed mutagenesis.
Nucleic acids research - 25 Jul 1990
Setzer D R, Hmiel R M, Liao S Y
Abstract excerpt
We describe a simple modification of commonly used single-stranded cloning vectors that permits the efficient recovery of mutant DNA molecules in oligonucleotide-directed mutagenesis experiments, even when the absolute efficiency of mutagenesis is very low. The modification consists of the insertion of a short synthetic DNA fragment into the vector's polylinker and permits the identification of mutant clones...
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