A simple vector modification to facilitate oligonucleotide-directed mutagenesis.

doi:10.1093/nar/18.14.4175

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Article

A simple vector modification to facilitate oligonucleotide-directed mutagenesis.

Nucleic acids research - 25 Jul 1990

Setzer D R, Hmiel R M, Liao S Y

Abstract excerpt

We describe a simple modification of commonly used single-stranded cloning vectors that permits the efficient recovery of mutant DNA molecules in oligonucleotide-directed mutagenesis experiments, even when the absolute efficiency of mutagenesis is very low. The modification consists of the insert...

Topics

Base Sequence
Escherichia coli
Genetic Vectors
Molecular Sequence Data
Mutation
Oligodeoxyribonucleotides
Transcription, Genetic
beta-Galactosidase

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