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Article

Oligonucleotide-directed mutagenesis without phenotypic selection.

Current protocols in neuroscience - 1 May 2001

Kunkel T A

Abstract excerpt

A DNA sequence can be specifically altered by synthesizing the desired sequence change within an oligonucleotide, and then converting this into a biologically active circular DNA strand by using the oligonucleotide to prime in vitro synthesis on a single-stranded circular template. This unit presents a protocol which uses a DNA template containing a small number of uracil residues in place of thymine. Use of the...

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PMID: 18428474
DOI: 10.1002/0471142301.ns0410s03

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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