In vivo site-specific mutagenesis and gene collage using the delitto perfetto system in yeast Saccharomyces cerevisiae.

doi:10.1007/978-1-61779-129-1_11

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Article

In vivo site-specific mutagenesis and gene collage using the delitto perfetto system in yeast Saccharomyces cerevisiae.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011

Stuckey Samantha, Mukherjee Kuntal, Storici Francesca

Abstract excerpt

Delitto perfetto is a site-specific in vivo mutagenesis system that has been developed to generate changes at will in the genome of the yeast Saccharomyces cerevisiae. Using this technique, it is possible to rapidly and efficiently engineer yeast strains without requiring several intermediate steps as it functions in only two steps, both of which rely on homologous recombination to drive the changes to the target...

Topics

DNA Breaks, Double-Stranded
Genotype
Mutagenesis, Site-Directed
Polymerase Chain Reaction
Saccharomyces cerevisiae

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Identifiers

PMID: 21660695
DOI: 10.1007/978-1-61779-129-1_11
PMCID: PMC4890625

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