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Article

Recombineering: highly efficient in vivo genetic engineering using single-strand oligos.

Methods in enzymology - 1 Jan 2013

Sawitzke James A, Thomason Lynn C, Bubunenko Mikhail, Li Xintian, Costantino Nina, Court Donald L

Abstract excerpt

Recombineering provides the ability to make rapid, precise, and inexpensive genetic alterations to any DNA sequence, either in the chromosome or cloned onto a vector that replicates in E. coli (or other recombineering-proficient bacteria), and to do so in a highly efficient manner. Complicated genetic constructs that are impossible to make with in vitro genetic engineering can be created in days with...

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Identifiers

PMID: 24182922
DOI: 10.1016/B978-0-12-420067-8.00010-6
PMCID: PMC7518103

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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