Article
Molecular genetic analysis of an endotoxin nonresponder mutant cell line: a point mutation in a conserved region of MD-2 abolishes endotoxin-induced signaling.
The Journal of experimental medicine - 2 Jul 2001
Schromm A B, Lien E, Henneke P, Chow J C, Yoshimura A, Heine H, Latz E, Monks B G, Schwartz D A, Miyake K, Golenbock D T
Abstract excerpt
Somatic cell mutagenesis is a powerful tool for characterizing receptor systems. We reported previously two complementation groups of mutant cell lines derived from CD14-transfected Chinese hamster ovary--K1 fibroblasts defective in responses to bacterial endotoxin. Both classes of mutants expressed a normal gene product for Toll-like receptor (TLR)4, and fully responded to stimulation by tumor necrosis factor...
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