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Article

Improved autoprocessing efficiency of mutant subtilisins E with altered specificity by engineering of the pro-region.

Journal of biochemistry - 1 Jul 2001

Takahashi M, Hasuura Y, Nakamori S, Takagi H

Abstract excerpt

Modification of substrate specificity of an autoprocessing enzyme is accompanied by a risk of significant failure of self-cleavage of the pro-region essential for activation. Therefore, to enhance processing, we engineered the pro-region of mutant subtilisins E of Bacillus subtilis with altered substrate specificity. A high-activity mutant subtilisin E with Ile31Leu replacement (I31L) as well as the wild-type...

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Identifiers

PMID: 11432785
DOI: 10.1093/oxfordjournals.jbchem.a002968

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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