Selection of Streptomyces griseus protease B mutants with desired alterations in primary specificity using a library screening strategy.

doi:10.1006/jmbi.1996.0159

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Article

Selection of Streptomyces griseus protease B mutants with desired alterations in primary specificity using a library screening strategy.

Journal of molecular biology - 29 Mar 1996

Sidhu S S, Borgford T J

Abstract excerpt

Streptomyces griseus protease B (SGPB) has primary specificity for large hydrophobic residues. The protease is secreted in a promature form, and autocatalytic removal of the propeptide is essential for activity. We genetically substituted the P1 Leu at the promature junction of SGPB with Phe, Met...

Topics

Amino Acids
Bacillus subtilis
Base Sequence
DNA Mutational Analysis
Enzyme Stability
Escherichia coli
Gene Library
Genetic Variation
Half-Life
Milk Proteins
Models, Molecular
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis

Mutation

Protein Precursors

Recombinant Fusion Proteins

Serine Endopeptidases

Streptomyces griseus

Source

Open in PubMed

Identifiers

PMID: 8609620
DOI: 10.1006/jmbi.1996.0159

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8609620-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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