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Article

Selection of Streptomyces griseus protease B mutants with desired alterations in primary specificity using a library screening strategy.

Journal of molecular biology - 29 Mar 1996

Sidhu S S, Borgford T J

Abstract excerpt

Streptomyces griseus protease B (SGPB) has primary specificity for large hydrophobic residues. The protease is secreted in a promature form, and autocatalytic removal of the propeptide is essential for activity. We genetically substituted the P1 Leu at the promature junction of SGPB with Phe, Met, or Val and monitored expression levels in Escherichia coli. Substitution with Phe had no effect on active SGPB...

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Identifiers

PMID: 8609620
DOI: 10.1006/jmbi.1996.0159

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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