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Article

Probing the altered specificity and catalytic properties of mutant subtilisin chemically modified at position S156C and S166C in the S1 pocket.

Bioorganic & medicinal chemistry - 1 Jul 1999

DeSantis G, Jones J B

Abstract excerpt

A series of chemically modified mutants (CMMs) of subtilisin B. lentus (SBL) were generated employing the combination of site-directed mutagenesis and chemical modification. This strategy entails the mutation of a selected active site residue to cysteine and its subsequent modification with a methanethiosulfonate reagent CH3SO2S-R, where R may be infinitely variable. The present study was undertaken to evaluate...

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Identifiers

PMID: 10465412
DOI: 10.1016/s0968-0896(99)00068-1

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