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Article

Enhanced thermostability of the single-Cys mutant subtilisin E under oxidizing conditions.

Journal of biochemistry - 1 Oct 2000

Takagi H, Hirai K, Wada M, Nakamori S

Abstract excerpt

We obtained enhanced thermostability by replacing Ser161 with Cys in subtilisin E from Bacillus subtilis, a cysteine-free alkaline serine protease. The Ser161Cys mutant subtilisin E was purified from the culture supernatant of the recombinant B. subtilis in an oxidizing environment. SDS-polyacrylamide gel electrophoresis and mass spectrometry under oxidizing conditions indicated that the mutant enzyme in part...

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PMID: 11011140
DOI: 10.1093/oxfordjournals.jbchem.a022790

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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