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Article

Contribution of Ser463 residue to the enzymatic and autoprocessing activities of Escherichia coli gamma-glutamyltranspeptidase.

Indian journal of biochemistry & biophysics - 1 Aug 2009

Hsu Wen-Hwei, Ong Ping-Lin, Chen Shih-Chun, Lin Long-Liu

Abstract excerpt

A serine residue Ser463, required for proper function of E. coli y-glutamyltranspeptidase (EcGGT) was identified by site-directed mutagenesis on the basis of sequence alignment of human, pig, rat, and three bacterial enzymes. Thr-, Asp-, and Lys-substituted variants were overexpressed in E. coli M15 cells and the recombinant proteins were purified to near homogeneity by nickel-chelate chromatography. With the...

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PMID: 19788059

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