Article
Prevention of C-terminal autoprocessing of Lactococcus lactis SK11 cell-envelope proteinase by engineering of an essential surface loop.
The Biochemical journal - 15 Sept 1994
Bruinenberg P G, de Vos W M, Siezen R J
Abstract excerpt
The catalytic domain of the cell-envelope proteinase from Lactococcus lactis SK11 has various inserts, situated in external loops of the catalytic domain, compared with the related subtilisins. Protein engineering was employed to analyse the necessity and function of one of these extra loops (res...
Topics
- Amino Acid Sequence
- Animals
- Bacterial Proteins
- Base Sequence
- Cell Membrane
- Cloning, Molecular
- Endopeptidases
- Enzyme Stability
- Lactococcus lactis
- Milk
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Protein Engineering
- Protein Processing, Post-Translational
- Protein Structure, Secondary
- Serine Endopeptidases
- Substrate Specificity