Article
RhD genotyping by quantitative fluorescent polymerase chain reaction: a new approach.
BJOG : an international journal of obstetrics and gynaecology - 1 Dec 2000
Pertl B, Pieber D, Panzitt T, Haeusler M C, Winter R, Tului L, Brambati B, Adinolfi M
Abstract excerpt
OBJECTIVE: To develop a new method of RhD/d genotype determination using a quantitative fluorescent PCR (QF-PCR) assay. METHODS: Polymerase chain reaction amplification (PCR) of fragments of exon 7 of both the RHD and RHCE genes was performed from 32 amniotic fluid and 26 chorionic villus samples known to be heterozygous for the RHD gene, 74 peripheral blood samples of RhD-positive blood donors (homozygous or...
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