Article
Genotyping of RHD by multiplex polymerase chain reaction analysis of six RHD-specific exons.
Transfusion - 1 Jan 2000
Maaskant-van Wijk P A, Faas B H, de Ruijter J A, Overbeeke M A, von dem Borne A E, van Rhenen D J, van der Schoot C E
Abstract excerpt
BACKGROUND: Qualitative RHD variants are the result of the replacement of RHD exons by their RHCE counterparts or of point mutations in RHD causing amino acid substitutions. For RHD typing, the use of at least two RHD typing polymerase chain reaction (PCR) assays directed at different regions of...
Topics
- Alleles
- Base Sequence
- DNA
- DNA Primers
- Exons
- Genotype
- Humans
- Nucleic Acid Amplification Techniques
- Point Mutation
- Polymerase Chain Reaction
- Rh-Hr Blood-Group System
- Sensitivity and Specificity