Article
Sensitive determination of the RhD genotype in mixed samples using fluorescence-based polymerase chain reaction.
Vox sanguinis - 1 Jan 1997
Tonn T, Westrup D, Seidl C, Kirchmaier C M, Seifried E
Abstract excerpt
OBJECTIVES: Prenatal determination of the fetal RhD status in pregnancies of Rh-negative (Rh-neg) women by polymerase chain reaction (PCR) on DNA has become of increasing importance. Since determination of the RhD genotype in these cases is usually performed in samples containing both maternal Rh-neg and fetal Rh-neg or Rh-positive (Rh-pos) DNA, the sensitivity and specificity of PCR-based DNA detection are of...
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