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Article

Reliability and clinical application of fetal RhD genotyping with two different fluorescent duplex polymerase chain reaction assays: three years' experience.

American journal of obstetrics and gynecology - 1 Feb 1999

Crombach G, Niederacher D, Larbig D, Picard F, Tutschek B, Beckmann M W, Bender H G

Abstract excerpt

OBJECTIVE: This study was designed to evaluate accuracy and clinical usefulness of fetal RhD genotyping with fluorescent duplex polymerase chain reaction. STUDY DESIGN: Two RhD-specific gene fragments (exon 10 in polymerase chain reaction 1 and exon 7 in polymerase chain reaction 2) were amplified in samples from 213 fetuses. RESULTS: Amplification failed in 0.9% of the specimens, and equivocal results were found...

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PMID: 9988815
DOI: 10.1016/s0002-9378(99)70228-0

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