Specificity and sensitivity of RHD genotyping methods by PCR-based DNA amplification.

doi:10.1046/j.1365-2141.1997.2193040.x

Back to search

Article

Specificity and sensitivity of RHD genotyping methods by PCR-based DNA amplification.

British journal of haematology - 1 Aug 1997

Aubin J T, Le Van Kim C, Mouro I, Colin Y, Bignozzi C, Brossard Y, Cartron J P

Abstract excerpt

We have compared the sensitivity and specificity of four PCR methods of RHD gene detection using different sets of primers located in the regions of highest divergence between the RHD and RHCE genes, notably exon 10 (method I), exon 7 (method II), exon 4 (method III) and intron 4 (method IV). Met...

Topics

Blotting, Southern
DNA
Gene Amplification
Genotype
Humans
Polymerase Chain Reaction
Prenatal Diagnosis
Rh Isoimmunization
Rho(D) Immune Globulin
Sensitivity and Specificity

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.