Article
The Tn7 transposase is a heteromeric complex in which DNA breakage and joining activities are distributed between different gene products.
The EMBO journal - 15 Nov 1996
Sarnovsky R J, May E W, Craig N L
Abstract excerpt
The bacterial transposon Tn7 translocates by a cut and paste mechanism: excision from the donor site results from double-strand breaks at each end of Tn7 and target insertion results from joining of the exposed 3' Tn7 tips to the target DNA. Through site-directed mutagenesis of the Tn7-encoded tr...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Binding Sites
- Cysteine
- DNA
- DNA Nucleotidyltransferases
- DNA Transposable Elements
- DNA, Superhelical
- DNA-Binding Proteins
- Electrophoresis, Agar Gel
- Integrases
- Manganese
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Retroviridae
- Sequence Alignment
- Transposases