Article
Rapid engineering of bacterial artificial chromosomes using oligonucleotides.
Genesis (New York, N.Y. : 2000) - 1 Jan 2001
Swaminathan S, Ellis H M, Waters L S, Yu D, Lee E C, Court D L, Sharan S K
Abstract excerpt
A rapid method obviating the use of selectable markers to genetically manipulate large DNA inserts cloned into bacterial artificial chromosomes is described. Mutations such as single-base changes, deletions, and insertions can be recombined into a BAC by using synthetic single-stranded oligonucleotides as targeting vectors. The oligonucleotides include the mutated sequence flanked by short homology arms of 35-70...
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