Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Rapid generation of random mutant libraries.

Bioengineered bugs - 1 Jan 2000

Abou-Nader Mary, Benedik Michael J

Abstract excerpt

A simple and efficient method utilizing in vivo recombination to create recombinant libraries incorporating the products of PCR amplification is described. This will be especially useful for generating large pools of randomly mutagenized clones after error-prone PCR mutagenesis. Here we investigate various parameters to optimize this approach and we demonstrate that as little as 1 pmole of PCR fragment can...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Polymerase Chain Reaction
Share to Transformation, Genetic
Share to Bacillus

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Gene Library

Share to Mutagenesis

Share to Mutation

Share to Plasmids

Source

Open in PubMed Open full text

Identifiers

PMID: 21326833
DOI: 10.4161/bbug.1.5.12942
PMCID: PMC3037583

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Rapid generation of random mutant libraries.PMID 21326833Read ↗

PCR-based random mutagenesis.Semantic proximity Read ↗

Random mutagenesis libraries: optimization and simplification by PCR.Semantic proximity Read ↗

Generating mutant libraries using error-prone PCR.Semantic proximity Read ↗

Production of randomly mutated plasmid libraries using mutator strains.Semantic proximity Read ↗

Error-prone rolling circle amplification: the simplest random mutagenesis protocol.Semantic proximity Read ↗

Random Mutagenesis Using Error-Prone DNA Polymerases.Semantic proximity Read ↗

A convenient and robust method for construction of combinatorial and random mutant libraries.Semantic proximity Read ↗

Random Mutagenesis by PCR.Semantic proximity Read ↗

Random mutagenesis using a mutator strain.Semantic proximity Read ↗

Polishing the craft of genetic diversity creation in directed evolution.Semantic proximity Read ↗

Recent advances in targeted mutagenesis to expedite the evolution of biological systems.Semantic proximity Read ↗

Easy preparation of a large-size random gene mutagenesis library in Escherichia coli.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.