Article
ESI mutagenesis: a one-step method for introducing mutations into bacterial artificial chromosomes.
Life science alliance - 1 Feb 2021
Rondelet Arnaud, Pozniakovsky Andrei, Namboodiri Devika, Cardoso da Silva Richard, Singh Divya, Leuschner Marit, Poser Ina, Ssykor Andrea, Berlitz Julian, Schmidt Nadine, Röhder Lea, Vader Gerben, Hyman Anthony A, Bird Alexander W
Abstract excerpt
Bacterial artificial chromosome (BAC)-based transgenes have emerged as a powerful tool for controlled and conditional interrogation of protein function in higher eukaryotes. Although homologous recombination-based recombineering methods have streamlined the efficient integration of protein tags onto BAC transgenes, generating precise point mutations has remained less efficient and time-consuming. Here, we present...
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