Article
Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69.
The Journal of biological chemistry - 30 Mar 2001
Bebenek A, Dressman H K, Carver G T, Ng S, Petrov V, Yang G, Konigsberg W H, Karam J D, Drake J W
Abstract excerpt
The DNA polymerases (gp43s) of the related bacteriophages T4 and RB69 are B family (polymerase alpha class) enzymes that determine the fidelity of phage DNA replication. A T4 whose gene 43 has been mutationally inactivated can be replicated by a cognate RB69 gp43 encoded by a recombinant plasmid in T4-infected Escherichia coli. We used this phage-plasmid complementation assay to obtain rapid and sensitive...
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