Article
Side chains that influence fidelity at the polymerase active site of Escherichia coli DNA polymerase I (Klenow fragment).
The Journal of biological chemistry - 29 Jan 1999
Minnick D T, Bebenek K, Osheroff W P, Turner R M, Astatke M, Liu L, Kunkel T A, Joyce C M
Abstract excerpt
To investigate the interactions that determine DNA polymerase accuracy, we have measured the fidelity of 26 mutants with amino acid substitutions in the polymerase domain of a 3'-5'-exonuclease-deficient Klenow fragment. Most of these mutant polymerases synthesized DNA with an apparent fidelity similar to that of the wild-type control, suggesting that fidelity at the polymerase active site depends on highly...
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