Article
Side chains that influence fidelity at the polymerase active site of Escherichia coli DNA polymerase I (Klenow fragment).
The Journal of biological chemistry - 29 Jan 1999
Minnick D T, Bebenek K, Osheroff W P, Turner R M, Astatke M, Liu L, Kunkel T A, Joyce C M
Abstract excerpt
To investigate the interactions that determine DNA polymerase accuracy, we have measured the fidelity of 26 mutants with amino acid substitutions in the polymerase domain of a 3'-5'-exonuclease-deficient Klenow fragment. Most of these mutant polymerases synthesized DNA with an apparent fidelity s...
Topics
- Amino Acid Substitution
- Binding Sites
- Catalysis
- DNA Polymerase I
- Escherichia coli
- Models, Molecular
- Mutagenesis, Site-Directed
- Phenotype
- Structure-Activity Relationship