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Article

RB69 DNA polymerase mutants with expanded nascent base-pair-binding pockets are highly efficient but have reduced base selectivity.

Biochemistry - 28 Jul 2009

Zhang Hong, Beckman Jeff, Wang Jimin, Konigsberg William

Abstract excerpt

We have investigated the effect of systematically enlarging the nascent base-pair-binding pocket (NBP) of a replicative DNA polymerase from bacteriophage RB69 (RB69 pol) on the incorporation efficiency (k(pol)/K(d,app)) for both correct and incorrect dNMPs. Accordingly, we replaced residues L561, Y567, and S565 in the NBP with Ala, Ala, and Gly, respectively. We combined L561A and Y567A to give a double mutant...

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Identifiers

PMID: 19522539
DOI: 10.1021/bi900422b
PMCID: PMC2847438

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Source receipt: pubmed, retrieved 7 Sept 2026.

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