Article
Regulation of B family DNA polymerase fidelity by a conserved active site residue: characterization of M644W, M644L and M644F mutants of yeast DNA polymerase epsilon.
Nucleic acids research - 1 Jan 2007
Pursell Zachary F, Isoz Isabelle, Lundström Else-Britt, Johansson Erik, Kunkel Thomas A
Abstract excerpt
To better understand the functions and fidelity of DNA polymerase epsilon (Pol epsilon), we report here on the fidelity of yeast Pol epsilon mutants with leucine, tryptophan or phenylalanine replacing Met644. The Met644 side chain interacts with an invariant tyrosine that contacts the sugar of the incoming dNTP. M644W and M644L Pol epsilon synthesize DNA with high fidelity, but M644F Pol epsilon has reduced...
Topics
- Amino Acid Substitution
- Binding Sites
- DNA
- DNA Polymerase II
- Exodeoxyribonucleases
- Leucine
- Methionine
- Mutation
- Phenotype
- Phenylalanine
- Saccharomyces cerevisiae