Article
Repair of chromosomal abasic sites in vivo involves at least three different repair pathways.
The EMBO journal - 16 Oct 2000
Otterlei M, Kavli B, Standal R, Skjelbred C, Bharati S, Krokan H E
Abstract excerpt
We introduced multiple abasic sites (AP sites) in the chromosome of repair-deficient mutants of Escherichia coli, in vivo, by expressing engineered variants of uracil-DNA glycosylase that remove either thymine or cytosine. After introduction of AP sites, deficiencies in base excision repair (BER) or recombination were associated with strongly enhanced cytotoxicity and elevated mutation frequencies, selected as...
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