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Article

Function-based isolation of novel enzymes from a large library.

Nature biotechnology - 1 Oct 2000

Olsen M J, Stephens D, Griffiths D, Daugherty P, Georgiou G, Iverson B L

Abstract excerpt

Here we describe a high-throughput, quantitative method for the isolation of enzymes with novel substrate specificities from large libraries of protein variants. Protein variants are displayed on the surface of microorganisms and incubated with a synthetic substrate consisting of (1) a fluorescent dye (2) a positively charged moiety (3) the target scissile bond, and (4) a fluorescence resonance energy transfer...

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Identifiers

PMID: 11017045
DOI: 10.1038/80267

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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