Article

A generalisable method for the purification and biophysical characterisation of bacterial membrane receptors

2026-08-12

Abstract excerpt

Membrane-embedded bacterial receptors are challenging to express and purify in soluble form, yet their isolated domains are essential tools for structural and ligand-discovery studies. Pseudomonas aeruginosa relies on the TonB-dependent heme receptor HasR for iron acquisition, a process central to its pathogenicity. Here, we report a robust strategy for the recombinant expression, purification, and biophysical ch...

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Literature Corpus work: a10d8005-7531-5d13-ab56-8b04efd2500d
DOI: 10.64898/2026.08.12.744447

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