Rapid isolation of high-affinity protein binding peptides using bacterial display

Abstract excerpt

A robust bacterial display methodology was developed that allows the rapid isolation of peptides that bind to arbitrarily selected targets with high affinity. To demonstrate the utility of this approach, a large library (5 x 10(10) clones) was constructed composed of random 15-mer peptide insertions constrained within a flexible, surface exposed loop of the Escherichia coli outer membrane protein A (OmpA). The...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Advanced Biosensing Techniques and Applications
• Share to Bacteriophages and microbial interactions
• Share to Monoclonal and Polyclonal Antibodies Research