Article

A FRET Ligation Assay using Fluorescent Proteins for Bacterial Sortase Enzymes

2026-08-24

Abstract excerpt

Bacterial sortases are widely used in sortase-mediated ligation (SML) experiments for various protein engineering applications. The power of these enzymes to bind and cleave a specific recognition motif, followed by ligation to another substrate using a ping-pong reaction mechanism has numerous applications in vaccine and antibody/nanobody drug conjugate development, as a diagnostic and therapeutic tool, in creati...

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Literature Corpus work: 9b52fae1-f7fe-5048-afab-eab2ecb0a6b3
DOI: 10.64898/2026.08.21.746329

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