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Article

A dominant negative mutation in a spliceosomal ATPase affects ATP hydrolysis but not binding to the spliceosome.

Molecular and cellular biology - 1 Aug 1992

Schwer B, Guthrie C

Abstract excerpt

PRP16 is an RNA-dependent ATPase required for the second catalytic step of splicing in vitro. A dominant suppressor of a branchpoint mutation in Saccharomyces cerevisiae, the prp16-1 allele, contains a Tyr to Asp change in the nucleotide-binding site consensus sequence. We now find that cells harboring the prp16-1 allele have a general growth defect that is exacerbated at cold temperatures. The mutant is dominant...

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Identifiers

PMID: 1385854
DOI: 10.1128/mcb.12.8.3540-3547.1992
PMCID: PMC364619

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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A dominant negative mutation in a spliceosomal ATPase affects ATP hydrolysis but not binding to the spliceosome.PMID 1385854Read ↗

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