Article
A dominant negative mutation in a spliceosomal ATPase affects ATP hydrolysis but not binding to the spliceosome.
Molecular and cellular biology - 1 Aug 1992
Schwer B, Guthrie C
Abstract excerpt
PRP16 is an RNA-dependent ATPase required for the second catalytic step of splicing in vitro. A dominant suppressor of a branchpoint mutation in Saccharomyces cerevisiae, the prp16-1 allele, contains a Tyr to Asp change in the nucleotide-binding site consensus sequence. We now find that cells har...
Topics
- Adenosine Triphosphatases
- Alleles
- Amino Acid Sequence
- Fungal Proteins
- Genes, Dominant
- Genes, Fungal
- Genetic Complementation Test
- Kinetics
- Mutation
- Phenotype
- RNA Helicases
- RNA Splicing
- RNA Splicing Factors
- RNA, Fungal
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins