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[Development of a mouse cell line containing stably integrated copies of pMCLacI/Neo plasmid: a model for studying mutations in vitro].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Apr 2000

Lu Y, Li H, Fu J

Abstract excerpt

OBJECTIVE: To establish a suitable model for studying the different mechanisms of mutation between expressed and non-expressed genes in mammalian cells. METHODS: The NIH3T3 cells were transfected with the linearized pMCLacI/Neo DNAs by liposome-mediated transfection, and grew in the presence of G418. One drug resistant cell clone was selected to proliferate and to be analyzed with Southern blot and RT-PCR...

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PMID: 10751538

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