[Construction of eukaryotic expression vector using neomycin-resistance gene mutant as selectable marker].

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Article

[Construction of eukaryotic expression vector using neomycin-resistance gene mutant as selectable marker].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 May 2002

Gao Chuan, Zhu Xu-Dong, Zhou Xiao-Wei, Yu Fang, Lu Bai-Song, Huang Pei-Tang

Abstract excerpt

Neomycin-resistance gene is widely used as a selectable marker in eukaryotic expression vector. It codes neomycin phosphotransferase II (NPT II) which confers resistance to various aminoglycoside antibiotic such as G418 and kanamycine. In this work, by site-directed mutagenesis the neo gene mutant was obtained. The expression vector pmDNA using the neo gene mutant as selectable marker has been constructed. After...

Topics

Amino Acid Sequence
Base Sequence
Drug Resistance
Genetic Markers
Genetic Vectors
Kanamycin Kinase
Molecular Sequence Data
Mutation

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Identifiers

PMID: 12192863

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