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Article

[Construction of eukaryotic expression vector using neomycin-resistance gene mutant as selectable marker].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 May 2002

Gao Chuan, Zhu Xu-Dong, Zhou Xiao-Wei, Yu Fang, Lu Bai-Song, Huang Pei-Tang

Abstract excerpt

Neomycin-resistance gene is widely used as a selectable marker in eukaryotic expression vector. It codes neomycin phosphotransferase II (NPT II) which confers resistance to various aminoglycoside antibiotic such as G418 and kanamycine. In this work, by site-directed mutagenesis the neo gene mutant was obtained. The expression vector pmDNA using the neo gene mutant as selectable marker has been constructed. After...

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PMID: 12192863

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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