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Article

RNA interference rescue by bacterial artificial chromosome transgenesis in mammalian tissue culture cells.

Proceedings of the National Academy of Sciences of the United States of America - 15 Feb 2005

Kittler Ralf, Pelletier Laurence, Ma Chunling, Poser Ina, Fischer Steffi, Hyman Anthony A, Buchholz Frank

Abstract excerpt

RNA interference (RNAi) is a widely used method for analysis of gene function in tissue culture cells. However, to date there has been no reliable method for testing the specificity of any particular RNAi experiment. The ideal experiment is to rescue the phenotype by expression of the target gene in a form refractory to RNAi. The transgene should be expressed at physiological levels and with its different splice...

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Identifiers

PMID: 15695330
DOI: 10.1073/pnas.0409861102
PMCID: PMC548992

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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