Article
[Construction of eukaryotic expression vector containing hSNCA gene and its pathogenic mutants and its expression in PC12 cells].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology - 1 Jan 2008
Qian Jin-jun, Cheng Yan-bo, Liu Chun-feng, Yang Ya-ping, Liu Kang-yong
Abstract excerpt
AIM: To construct recombinant eukaryotic expression vectors pEGFP-C3-SNCA containing human wild-type (WT) and pathogenic mutations A30P, A53T alpha-synuclein (SNCA), and to obtain monoclonal PC12 cell lines overexpressing human wild-type and pathogenic mutations A30P, A53T alpha-synuclein by stab...
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