Article
[Construction and identification of primary open angle glaucoma disease MYOC Pro356Leu mutant plasmid in mouse].
Yan ke xue bao = Eye science - 1 Aug 2010
Li Chunmei, Zhuo Yehong, Chen Mengfei, Sun Xuerong, Qi Ying, Ge Jian
Abstract excerpt
PURPOSE: To construct and identify the plasmid of mutant MYOC Pro356Leu in mouse. METHODS: 1.Transfering Red recombinantinase- expressing plasmid pKD46 was transferred to RP23-180F15 BAC clone by electroporation.2.Amplifying tThe pStart-K vector was amplified by PCR and electroporating the PCR product was electroporated into the bacteria containing the mMYOC. 3.PCR amplification of the mMyoc AfeI fragment and...
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