Article
[Comparative study of eukaryotic cell expression of wild-type and Pro370Leu mutation type myocilin gene].
Zhonghua yi xue za zhi - 14 Mar 2006
Wang Jian-wen, Sun Hui-min, Ji Jian, Li Xiao-rong, Zheng Jian-qiu
Abstract excerpt
OBJECTIVE: To compare the eukaryotic cell expression of wild-type myocilin (MYOC) (wMYOC) gene and Pro370Leu mutation type MYOC (mMYOC) gene so as to understand the mechanism of primary open angle glaucoma (POAG). METHODS: HeLa cells were cultured and then transfected with the vector pEGFP-N3-wMYOC, recombinant plasmid with wild type MYOC gene and enhanced green fluorescein gene, or the vector pDsRed2-N1-mMYOC,...
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