Construction of a neo fusion gene for expression in both prokaryotic and eukaryotic cells.

doi:10.1016/0378-1119(90)90044-r

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Article

Construction of a neo fusion gene for expression in both prokaryotic and eukaryotic cells.

Gene - 16 Apr 1990

Harwood A J, Shervington A, Bostock C J

Abstract excerpt

A high-copy-number plasmid, pLink, was constructed to allow the direct selection in Escherichia coli of a neo fusion gene capable of conferring Geneticin (G418) resistance on mouse L cells. pLink was derived from pdMmtneo by insertion of a KpnI linker within the 5'-coding region of the neo gene....

Topics

Animals
Base Sequence
Drug Resistance, Microbial
Escherichia coli
Gentamicins
Humans
Hypoxanthine Phosphoribosyltransferase
Mice
Molecular Sequence Data
Mutation
Neomycin
Peptide Chain Termination, Translational
Plasmids
Recombinant Fusion Proteins
Selection, Genetic

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